Mitogen Induced Cell-mediated Cytotoxicity (MICC) in Human Peripheral Blood Granulocytes and Lymphocytes

Human Peripheral Blood Granulocytes

A central problem of the current studies on protooncogenes (cellular oncogenes, c-onc) is their role in the control of growth and differentiation of normal cells. The cfos protooncogene has been widely investigated and it has been demonstrated that cfos specific transcripts are induced in proliferating cells (1, 2) and during differentiation (3-6) . In particular, when HL60 (promyelocytic) and ...

Phosphoinositide hydrolysis in mitogen-stimulated human peripheral-blood T lymphocytes.

Both phytohaemagglutinin and antibodies to the CD3 molecule induced proliferation and phosphoinositide hydrolysis in human peripheral-blood T lymphocytes, but the magnitude of the inositol phosphate response was small and the rate of accumulation slow [significant increases in Ins(1,4,5)P3 were observed only after 10 min]. Hence this response differs from the well-characterized Ins(1,4,5)P3 res...

Effect of trolox and quercetin on sulfur mustard-induced cytotoxicity in human peripheral blood lymphocytes

Objective: To evaluate the protective activity of antioxidants, viz. trolox and quercetin, against sulfur mustard (SM)-induced cytotoxicity. Materials and Methods: Cytotoxicity of various concentrations (20–640 μM) of SM, in the Division of Pharmacology presence or absence of 10 μM trolox or quercetin (-0.5, 0, or +0.5 h) was determined in and Toxicology, Defence Research and Development human ...

Nonspecific cell-mediated cytotoxicity of peripheral blood lymphocytes derived from suckling piglets.

Cell-mediated immune response to bacterial products in human tonsils and peripheral blood lymphocytes.

Lymphoproliferative responses of tonsillar tissue lymphocytes and peripheral blood lymphocytes to phytohemagglutinin and specific bacterial product antigens were studied in children undergoing tonsillectomy and adenoidectomy. Tonsillar tissue lymphocytes responded to optimal concentrations of phytohemagglutinin. Varidase, and streptolysin-O in a manner similar to peripheral blood lymphocytes. H...